Fig. 1: VEGF-A splicing-sensitive fluorescent reporter design. | Oncogenesis

Fig. 1: VEGF-A splicing-sensitive fluorescent reporter design.

From: A drug-repositioning screen using splicing-sensitive fluorescent reporters identifies novel modulators of VEGF-A splicing with anti-angiogenic properties

Fig. 1

A Endogenous VEGF-A splicing patterns; terminal exon 8 alternative 3′ splicing results in the anti-angiogenic “b” isoforms. B Parts of VEGF-A gene (intron 7 and relevant regions of exon 8) have been inserted into the bichromatic reporter in such a way that splicing at the proximal splice site (PSS) of exon 8 results in formation of dsRED and then a stop codon is encountered, so EGFP is not formed. When the distal splice site (DSS) is used, there is a shift in the reading frame (dsRED + 1) and a fusion with EGFP is formed.

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