Fig. 3: Screening the LOPAC library for small molecules that effect the alternative splicing of the VEGF-A reporter construct. | Oncogenesis

Fig. 3: Screening the LOPAC library for small molecules that effect the alternative splicing of the VEGF-A reporter construct.

From: A drug-repositioning screen using splicing-sensitive fluorescent reporters identifies novel modulators of VEGF-A splicing with anti-angiogenic properties

Fig. 3

PC3 cells transfected with the fluorescent VEGF splicing reporter were treated with the LOPAC compound library and reporter splicing measured using a fluorescent plate reader (Primary screen, step 1). Hit compounds were used to treat non-splicing control reporter constructs (Control screen, step 2). Flow cytometry was used to measure the effect of the hit compounds on reporter splicing (Secondary screen, step 3).

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