Fig. 4: Elevated apoptosis and active caspase-3 in the intestinal tissues of Uhrf1 ^ki/ki/Apc^min/+ mice.

A, B TUNEL assay showing levels of apoptosis in the intestinal sections from different mice. Representative confocal microscope images of TUNEL assay (A). Nuclei were stained with DAPI. Scale bar, 25 μm. Quantitative results of TUNEL-positive cells per 0.01 mm² (B). Three mice were analyzed for each genotypes. Apc^min/+-Tumor, tumor samples from Uhrf1^+/+/Apc^min/+ and Uhrf1^ki/ki/Apc^min/+ mice. Error bars, S.E. C, D Representative immunohistochemistry images showing the cleaved-caspase-3 (cl-CASP3) levels in small intestinal tissue sections from different mice (C). Scale bar, 50 μm. The number of cl-CASP3-positive cells were analyzed by ImageJ software (D). Three mice were analyzed for each genotypes. Error bars, S.E. Western blot results showing the levels of cl-CASP3 (E) and full-length caspase-3 (F-CASP3) (F) in small intestinal epithelium cells from different mice as indicated. The relative levels of cl-CASP3 and F-CASP3 were determined by ImageJ software. G Western blot results showing the levels of cleaved PRAP (cl-PARP) and full-length PARP (F-PARP) proteins in small intestinal epithelium cells from different mice as indicated. Note elevated levels of cl-PARP proteins were observed in small intestinal epithelium cells from Uhrf1^ki/ki/Apc^min/+ mice. H Western blot results showing the levels of cleaved-caspase-3 (cl-CASP3) proteins in liver tissues and small intestine epithelium cells from different mice as indicated. Note that cl-CASP3 was not detected in liver tissues from Uhrf1^ki/ki/Apc^min/+ mice.