Fig. 3: CtBP1/2 knockdown increased the sensitivity to DNA damage response in ovarian cancer cells. | Oncogenesis

Fig. 3: CtBP1/2 knockdown increased the sensitivity to DNA damage response in ovarian cancer cells.

From: CtBP1/2 differentially regulate genomic stability and DNA repair pathway in high-grade serous ovarian cancer cell

Fig. 3

The upregulated genes in CtBP2 KD vs. control were enriched in DNA damage response as assessed by GSEA (A). Heat maps significantly compared expression of genes that involved in DNA damage response regulation in CtBP1/2 KD and control groups (B). Comet assay analyzed the DNA damage response the after g-irradiation treatment (6 and 12 Gy) on ovarian cancer cells. The ratio of tail DNA was as quantitative analysis (C). Immunofluorescence of γH2AX and RPA32 phosphorylation detected in control and CtBP1/2 KD cells after cell treated with carboplatin and etoposide (D). Nuclear DNA was counterstained with DAPI. Quantitative analysis foci formation per cell in each group was performed by Image J and presented as γH2AX foci/cell (E) and RPA32 foci/cell (F). Rest represented without stimulation. For each sample, 100 cells were blinded and scored independently by two markers. *P < 0.05; **P < 0.01; ***P < 0.001.

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