Fig. 4: CtBP1/2 KD significantly decreased the length of nascent DNA strands at stalled replication forks.

Schematic of single DNA fiber analysis experimental design. Green tracts, IdU; red tracts, CldU. Examples of various types of tracts are shown (A). The retention of IdU label was measured, with or without HU treatment, and reflected the stability of stalled forks (Σ IdU). The lengths of CldU tract formed after breakage have been measured (ΣCIdU), with or without HU exposure, to evaluate the impact of CtBP1/2 knockdown on the DNA replication recover ability in serous ovarian cancer cells. Demonstration of representative images of ldU/CldU tract, with or without HU treatment, in the control, CtBP1 KD, and CtBP1 KD groups (B). IdU tract length distributions from DNA fibers from control and CtBP1/2 KD cells in the presence (replication stalling) (C) or absence (unperturbed replication) of HU (D). CIdU tract length distributions from DNA fibers from control and CtBP1/2 KD cells in the presence (replication stalling) (E) or absence (unperturbed replication) of HU (F). Distribution curves of the ratio of CldU/IdU tract lengths with (G) or without HU in cells (H). Median tract lengths and cumulative distributions were given in parentheses here and in subsequent figures. Error bars represented the standard error of the mean (SEM).