Fig. 2: BTK-positive NSCLC cells possess the characteristics of stem cells and epithelial–mesenchymal transition (EMT). | Oncogenesis

Fig. 2: BTK-positive NSCLC cells possess the characteristics of stem cells and epithelial–mesenchymal transition (EMT).

From: Bruton’s tyrosine kinase (BTK) mediates resistance to EGFR inhibition in non-small-cell lung carcinoma

Fig. 2

A Representative flow-cytometry plots of BTK for A549 cells showing the gating strategy for sorting. The efficiency was confirmed by flow cytometry following the sorting of BTKpos and BTKneg cells from A549 and PC-9 lung cancer cells. B Representative images of spheres (scale bar 50 μm) and C quantification of spheres in A549 and PC-9 lung cancer cells sorted for BTK. D Migration and invasion were increased in BTKpos cells, as compared with BTKneg cells, in both A549 and PC-9 cells. Scale bar 20 μm. E RNA expression of cancer stemness and EMT markers in BTKpos cells and BTKneg cells. F Protein expression of cancer stemness and EMT markers in BTKpos cells and BTKneg cells. *p < 0.05, **p < 0.01.

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