Fig. 4: BTK is required for Gefitinib-induced cell death probably regulated by stemness. | Oncogenesis

Fig. 4: BTK is required for Gefitinib-induced cell death probably regulated by stemness.

From: Bruton’s tyrosine kinase (BTK) mediates resistance to EGFR inhibition in non-small-cell lung carcinoma

Fig. 4

A Cell viability evaluated by SRB assay in PC-9/GR, PC-9/GR/shCTL, PC-9/GR/shBTK-1, and PC-9/GR/shBTK-2 cells. B Cell viability evaluated by SRB assay in PC-9 (wild type), PC-9/pMCS-N1 (vector control), and PC-9/pBTK-N1 (BTK overexpression) cells. C Cell apoptosis determined by flow cytometry after treatment of 5 μM Gefitinib in PC-9/GR, PC-9/GR/shCTL, PC-9/GR/shBTK-1, and PC-9/GR/shBTK-2 cells. D Cell apoptosis determined by flow cytometry after treatment of 5 μM Gefitinib in PC-9, PC-9/pMCS-N1, and PC-9/pBTK-N1 cells. E mRNA expression of KLF4, SOX2, and NANOG according to PC-9 cells with differed status of BTK expression and Gefitinib resistance. Total RNA was harvested for the analysis of mRNA by real-time RT-PCR. F CD133 + cells detected by flow cytometry according to PC-9 cells with different status of BTK expression and Gefitinib resistance. The results are shown as the means ± SD of three independent experiments, each performed in triplicate. CTL control, *p < 0.05.

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