Fig. 5: BTK inhibitor Acalabrutinib induces apoptosis in A549 (EGFR wild type), H1975 (EGFR T790M), and PC-9 (mutant EGFR with 19 Del) cells.

A Effects of Acalabrutinib on apoptosis induction after treatment of Acalabrutinib at the indicated concentrations for 48 h. Apoptosis was determined by the annexin-V/PI assay. Bars represent mean ± SD. *p < 0.05, **p < 0.01. B Modulation of Acalabrutinib on apoptotic proteins in Gefitinib-resistant H1975 cells.