Fig. 3: Hypermethylated binding motif repels E2F1 from the ESRP1 promoter in hypoxic breast cancer increased DNA methylation. | Oncogenesis

Fig. 3: Hypermethylated binding motif repels E2F1 from the ESRP1 promoter in hypoxic breast cancer increased DNA methylation.

From: E2F1 and epigenetic modifiers orchestrate breast cancer progression by regulating oxygen-dependent ESRP1 expression

Fig. 3

A Immunoblots of E2F1, ESRP1, and HIF1α in MCF7 and HCC1806 cells (Normoxia versus Hypoxia). B–D Densitometric analysis of representative blots. E ChIP qRT-PCR on ESRP1 promoter using E2F1 antibody in HCC1806 cells (Normoxia versus Hypoxia). F MeDIP of DNA isolated from MCF7 cells (Normoxia versus Hypoxia) using 5-methyl-cytosine antibody followed by qRT-PCR, relative to input and control IgG (n = 3). G MeDIP on ESRP1 promoter using 5-methyl-cytosine antibody after treating MCF7 cells with 5-aza-2’-deoxycytidine under hypoxia followed by qRT-PCR, relative to input and control IgG (n = 3). H ChIP qRT-PCR on ESRP1promoter using E2F1 antibody in 5-aza-2’-deoxycytidine (10 μM) treated MCF7 cells under hypoxia. I Immunoblots of DNMT1, DNMT3A, DNMT3B, and ESRP1 protein expression in shDNMT1, shDNMT3A, shDNMT3B, and shcontrol MCF7 cells under hypoxic condition. J, K Densitometric analysis of representative blots compared to shControl normalized to one. Error bars show mean values ± SD (n = 3 unless otherwise specified) calculated using two-tailed Student’s t test, **P < 0.01 and ***P < 0.001.

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