Fig. 5: E2F1 alters the cancer spliceome by inducing splicing factor SRSF7 expression in hypoxic breast cancer cells. | Oncogenesis

Fig. 5: E2F1 alters the cancer spliceome by inducing splicing factor SRSF7 expression in hypoxic breast cancer cells.

From: E2F1 and epigenetic modifiers orchestrate breast cancer progression by regulating oxygen-dependent ESRP1 expression

Fig. 5

A Immunoblots of SRSF7 in MCF7 and HCC1806 cells (Normoxia versus Hypoxia). B Densitometric analysis of representative blots. C Immunoblots for E2F1 and SRSF7 in E2F1 knockout MCF7 and HCC1806 cells under hypoxia. D, E Densitometric analysis of representative blots. F ChIP qRT-PCR on SRSF7 promoter using E2F1 antibody in HCC1806 cells under hypoxia. Fold enrichment (E2F1/IgG) was normalized to 5% input. G Semi-quantitative PCR of FAS and Tau genes after 48 h of hypoxic treatment and E2F1 knockout under hypoxia in MCF7 and HCC1806 cells (RPS16 used as a control). **P < 0.01 and ***P < 0.001.

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