Fig. 3: 4.1R deficiency enhanced cell proliferation and reduced exhaustion of CAR T cells. | Oncogenesis

Fig. 3: 4.1R deficiency enhanced cell proliferation and reduced exhaustion of CAR T cells.

From: Suppression of 4.1R enhances the potency of NKG2D-CAR T cells against pancreatic carcinoma via activating ERK signaling pathway

Fig. 3

a Mock T, NKG2D-CAR T, NC-NKG2D-CAR T, and KD2-NKG2D-CAR T, labeled with CFSE, were co-incubated with PANC28 at a 1:5 ratio for 7 days. CFSE dilution was used as a measure of cell proliferation (left), and MFI was calculated (right) (n = 3). b, c Mock T, NKG2D-CAR T, NC-NKG2D-CAR T, and KD2-NKG2D-CAR T were co-incubated with PANC28 at a 1:5 ratio for 3 days. Anti-CD3 staining was used to distinguish T cells from target cells. The expression of TIM-3 and PD-1 was measured by flow cytometry (left). MFI and percentage were statistically analyzed and shown in column chart (middle and right) (n = 3). Data were representative of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, NS not significant.

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