Fig. 5: 4.1R deficiency showed effective and persistent anti-tumor activity against xenografts formed by PANC28 in mice.

a Schematic diagram of a complete animal experiment. b, c Tumor bioluminescence images of mice transplanted with PANC28/luc cells at the indicated time points. d Overall survival of mice was presented in Kaplan–Meier curves. e Line plots displayed the body weight of mice. f The counts of CD3+ T cells in peripheral circulation were measured by flow cytometry (left), and the percentage of CD3+ T cells was calculated (right) (n = 3). g The ratio of the subpopulation of NKG2D-CAR T cells in peripheral circulation was measured by flow cytometry. Data were representative of three independent experiments. **P < 0.01, ***P < 0.001, ****P < 0.0001, NS not significant.