Fig. 1: Depletion of NKX6.3 aberrantly regulates DNA replication. | Oncogenesis

Fig. 1: Depletion of NKX6.3 aberrantly regulates DNA replication.

From: Depletion of NK6 Homeobox 3 (NKX6.3) causes gastric carcinogenesis through copy number alterations by inducing impairment of DNA replication and repair regulation

Fig. 1

A Schematic of the general design of DNA fiber analysis. Depletion of NKX6.3 enhanced frequencies of stalled forks and newly-initiated forks (New origins; n = 3). B Representative images of DNA spreading. Lengths of IdU and CIdU tracts from DNA fibers were measured, showing the speed of fork progression and fork asymmetry in HFE-145shCtrl, HFE-145shNKX6.3#1, and HFE-145shNKX6.3#2 cells (n > 115). C S phase progression in HFE-145shCtrl, HFE-145shNKX6.3#1, and HFE-145shNKX6.3#2 cells (n = 3). D In immunoblot analysis, NKX6.3 depletion reduced expression of RPA1 but increased CDT1, Orc1, Cdc6, MCM2, MCM3, and MCM7 expression. E Schematic showing the CDT1 and RPA1 promoter and the NKX6.3 target regions. F, G ChIP-qPCR (left), real-time qPCR (middle), and immunoblot analysis (right) of CDT1 (F) and RPA1 (G) in HFE-145shCtrl, HFE-145shNKX6.3#1, and HFE-145shNKX6.3#2 cells (n = 3).

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