Fig. 3: NKX6.3 expression was correlated with CDT1 and RPA1 in a xenograft model and human gastric cancers. | Oncogenesis

Fig. 3: NKX6.3 expression was correlated with CDT1 and RPA1 in a xenograft model and human gastric cancers.

From: Depletion of NK6 Homeobox 3 (NKX6.3) causes gastric carcinogenesis through copy number alterations by inducing impairment of DNA replication and repair regulation

Fig. 3

A Tumor volume in mice implanted with HFE-145shNKX6.3#1 and HFE-145shNKX6.3#2 cells, whereas all mice subcutaneously injected with HFE-145shCtrl cells did not develop tumors (each group; n = 5). B Immunoblot analysis of RPA1, CDT1, Orc1, Cdc6, and γH2AX in tumors derived from mice implanted with HFE-145shNKX6.3#1 and HFE-145shNKX6.3#2 cells. C In immunoprecipitation analysis, the failure of the MRN complex formation in tumors derived from mice implanted with HFE-145shNKX6.3#1 and HFE-145shNKX6.3#2 cells. D, E The expression levels of NKX6.3, CDT1, and RPA1 mRNA (D) and protein (E) in 60 gastric cancers. F NKX6.3 expression was inversely correlated with CDT1 (left) and positive correlated with RPA1 (right) in non-cancerous gastric mucosae (upper) and gastric cancers (lower; n = 60). G The schematic model hypothesized that NKX6.3 depletion leads to DNA replication stress and DNA damage by transcriptional deregulation of CDT1 and RPA1.

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