Fig. 1: Human PanNETs are dependent on Cdk5.
From: Cdk5 drives formation of heterogeneous pancreatic neuroendocrine tumors
A H&E stain and immunostains for Cdk5 and p35/p25 in G1 PanNETs. Scale bars = 50 μm. Immunostains for Cdk5 (B) and p35/25 (C) from human PanNET TMA. Scale bar = 2 mm. Semi-quantitation of Cdk5 expression from B (D) and p35/25 expression from C (E) normalized to expression levels of each in a normal placenta core; column 1 row 4 of the TMA. Map of TMA in Supplementary Table S1. F Immunoblot of Cdk5 pathway components in fibroblasts and PanNET cells. G–J PanNET cell lines were treated with increasing concentrations of Indo A and monitored for effects on cell viability (n = 3). K IC50 values obtained from viability assays in G–J and Supplementary Fig. S1; ANOVA with Tukey’s multiple comparisons test for IC50 values. All error bars represent SEM.
