Fig. 3: Downregulation of CCND3 is toxic for B-ALL cells.

A Efficiency of the shRNA-dependent CCND3 knockdown was assessed by immunoblot. Image is representative of n = 3. B B-ALL cell lines were transduced with plasmids expressing RFP and either a non-targeting control, or CCND3-targeting shRNAs. Data shown as mean ± SD, n = 3. Statistical analysis was performed with student’s t-test. *=p < 0.05. C Immunoblot shows downregulation of CCND3 expression by specific shRNA in BCR-ABL1 transformed mouse pre-B-cells. Image representative of n = 3. D shRNA-dependent knockdown of Ccnd3 in BCR-ABL1 transformed murine pre-B-cells significantly inhibits their fitness in a competitive growth assay. Data shown as mean ± SD, n = 3. Statistical analysis was performed by student’s t-test. **=p < 0.01. E B-ALL PDXs JFK125R, PDX2, and BLQ5 were transduced with vectors expressing a shRNA targeting CCND3. CCND3 downregulation was analyzed by immunoblot. Image representative of n = 3. F The cytotoxic effect of CCND3 depletion was analyzed by competitive growth test as described in legend to Fig. 3B. Data shown as mean ± SD, n = 3. Statistical analysis was performed by student’s t-test. *=p < 0.05 **=p < 0.01.