Fig. 2: Myeloma PCs activate expression of CXCL12 and CX43 in HS-5 cell line.
From: CXCL12/CXCR4 axis supports mitochondrial trafficking in tumor myeloma microenvironment
A Representative confocal microscopy images show that mitochondria were transferred to U266 cells via Tunneling Nanotubes (TNT), extracellular vesicles (EV), and cell-to-cell contact. Bar graphs show the % of events mediated by TNT, EVs, or cell-to-cell contact over the total number of transfer events observed. B mRNA expression of CXCL12 was evaluated in HC-MSCs (n = 6) and MM-MSCs (n = 10). C Western blot analysis of CXCL12 expression in MSC from HC- and MM-patients. β-actin protein was used as total protein loading reference. For analysis, the optical density of the bands was measured using Scion Image software. D The left panel shows representative pictures of HS-5 cells cultured alone or with NCI-H929 or U266 cell lines; cells were stained for CXCL12 (red) and CX43 (white). Quantification of CX43 MFI and of the colocalization index was reported. Bars indicate the standard error means (**p < 0.01; ***p < 0.001; ****p < 0.0001).
