Fig. 1: PSMC2 was upregulated in gastric cancer and the role of PSMC2 in gastric cancer cells was studied. (AGS and MGC-803 cells).
From: PSMC2 promotes the progression of gastric cancer via induction of RPS15A/mTOR pathway
A Immumohistochemical staining was conducted to evaluate the expression of PSMC2 in the gastric cancer tissues and para-carcinoma tissues. B Kaplan–Meier was performed to analyze the relationship between PSMC2 expression and overall survival rate of patients with gastric cancer. C The results of MTT assay showed that after PSMC2 was knocked down, the proliferation fold of gastric cancer cells was significantly reduced. D The results of clone formation assay indicated that downregulation of PMSC2 inhibited the clone formation ability of gastric cancer cells (AGS and MGC-803). E Flow cytometry was performed to evaluate the apoptosis level of gastric cancer cells infected with shPSMC2 or shCtrl lentivirus. F Transwell assay was conducted to determine the changes in the migration ability of gastric cancer cells after knocking down PSMC2. G Wound healing assay was conducted to determine the changes in the migration ability of gastric cancer cells after knocking down PSMC2. *P < 0.05; **P < 0.01; ***P < 0.001, compared with shCtrl group. Error bars meant SD.
