Fig. 5: PSMC2 regulated the expression of RPS15A through competitive binding with hsa-let-7c-3p. (MGC-803).

A Venny online tool was used to intersect the miRNAs targeted by PSMC2 and the miRNAs targeted by RPS15A. B The levels of PSMC2 mRNA and hsa-let-7c-3p in MGC-803 cells were determined by qPCR after PSMC2 knockdown. C Schematic diagram of the binding of hsa-let-7c-3p to h-PSMC2-3’UTR target site, and the mutation sequence of the PSMC2 gene. D The dual-luciferase reporter assay was conducted to detect the interaction between hsa-let-7c-3p and PSMC2. E Schematic diagram of the binding of hsa-let-7c-3p to h-RPS15A-3’UTR target site, and the mutation sequence of the RPS15A gene. F The dual-luciferase reporter assay was performed to detect the interaction between hsa-let-7c-3p and RPS15A. G The apoptosis of MGC-803 cells after inhibiting hsa-let-7c-3p or downregulating PSMC2 was determined by flow cytometry. H Transwell assay were conducted to determine the changes in the migration ability of MGC-803 cells after inhibiting hsa-let-7c-3p or downregulating PSMC2. *P < 0.05, **P < 0.01, ***P < 0.001, compared with shCtrl+Control group. ^#P < 0.05, ^##P < 0.01, ^###P < 0.001, compared with hsa-let-7c-3p inhibitor or PSMC2 knockdown group. Error bars meant SD.