Fig. 1: Characterization of AOS.

A The composition of AOS was identified by TLC assay. B Size exclusion chromatography was performed using an ÄKTA purifier system. The prepared alginate oligosaccharides were loaded onto a Sephadex G-15 column monitored at 235 nm, which was eluted with 0.2 M ammonium bicarbonate at a flow rate of 0.3 ml/min. C Schematic representation of the molecular structure of AOS and sulfated AOS (AOS-SO4) prepared by enzymatic degradation.