Fig. 4: AOS-SO4 promotes autophagy in osteosarcoma cells. | Oncogenesis

Fig. 4: AOS-SO4 promotes autophagy in osteosarcoma cells.

From: Sulfated alginate oligosaccharide exerts antitumor activity and autophagy induction by inactivating MEK1/ERK/mTOR signaling in a KSR1-dependent manner in osteosarcoma

Fig. 4

A After treatment with AOS-SO4 for 48 h, the protein levels of LC3B, ATG5, and ATG7 were assayed by western blot in osteosarcoma cells. B In parallel, autophagosome-like structures (indicated by the yellow arrows) were assayed by TEM. C LC3 puncta were analyzed by the mRFP-GFP-LC3 construct. D Osteosarcoma cells were treated with AOS-SO4 for 48 h with or without 3-MA (0.5 mM, 2 h), and flow cytometry was used to analyze apoptosis. E Representative blots of LC3B, ATG7, and Casp8 in MNNG, MG63, and U2OS cells after treated with AOS-SO4 for 48 h with or without 3-MA. Data are shown as the means ± SD from at least three independent experiments. Statistical analysis was performed using Student’s t-test. Error bars represent the SEM. *P < 0.05; **P < 0.01; ***P < 0.001.

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