Fig. 6: AOS-SO4 inhibits osteosarcoma cell growth through the MEK1/ERK/mTOR pathway in vivo.

A Photograph of tumor-bearing mice and photograph of tumors after the mice were euthanized in the control group, AOS-SO4 group and AOS-SO4 + THBQ group. B Growth curve drawn by measuring tumor volumes on the indicated days. C Diagram showing the tumor weights in the subcutaneous tumor model. D Diagram showing the mouse weight in the subcutaneous tumor model. E, F Representative images of p-ERK and LC3B staining in the subcutaneous tumor model. G The quantification of IHC staining about the protein expression of p-ERK, LC3B, CDK6, and Casp9. H Protein levels of proliferation, apoptosis, autophagy, and phosphorylated ERK and mTOR in tumor tissues in the three groups. Statistical analysis was performed using Student’s t-test. Error bars represent the SEM. *P < 0.05.