Fig. 2: GSK3β palmitoylation regulates the EZH2–STAT3 axis.

A Statistical analysis of Figure A showed a positive correlation between p-STAT3 (Y705) level and ZDHHC4 expression. Data are shown as means ± SD (n = 3). P-values were determined by two-tailed Student’s t-test. *P < 0.05; **P < 0.01; ***P < 0.001. B Western blot analysis of the phosphorylation activity of GSK3β, EZH2, and STAT3 after treatment with ZDHHC4 siRNA. C Immunoprecipitation was used to detect the interaction between GSK3β, EZH2, and STAT3 in SF126 and U118 cells. D Schematic diagram of GSK3β regulating the EZH2–STAT3 axis.