Fig. 4: GSK3β palmitoylation contributes to the maintenance of stemness of TMZ-resistant GBM cells. | Oncogenesis

Fig. 4: GSK3β palmitoylation contributes to the maintenance of stemness of TMZ-resistant GBM cells.

From: GSK3β palmitoylation mediated by ZDHHC4 promotes tumorigenicity of glioblastoma stem cells in temozolomide-resistant glioblastoma through the EZH2–STAT3 axis

Fig. 4

A Representative images of GSCs induced from SF126R with ZDHHC4 knockdown. B Limiting dilution assay analysis (ELDA) of GSCs showed the frequencies of neurosphere formation. The significance of the difference between the indicated groups was determined by the χ2 test (n = 3 independent experiments). C Left, Real-time PCR analysis shows mRNA levels of STAT3 target stem-cell markers in ZDHHC4-knockdown SF126R GSCs. Control-shRNA cells were set to 1; right, Western blot verifies the effect of ZDHHC4 knockdown. D Left, SF126R cells with stable ZDHHC4 knockdown are supplemented with GSK3β or STAT3 for 24 h. Cell viability is measured by CCK-8. The cells stably expressing shNC transfected with empty vector were set to 1; Right, Expression verification of GSK3β and STAT3. Data are shown as means ± SD (n = 3). P-values were determined by two-tailed Student’s t-test. *P < 0.05; **P < 0.01; ***P < 0.001.

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