Fig. 5: GBM with ZDHHC4 knockdown in vivo was more sensitive to TMZ treatment.
A U118R cells (shNC, shZDHHC4-1, and shZDHHC4-2, n = 5/group) (5 × 105 cells/mouse) were injected into nude mice. Mice were sacrificed 45 days later. H&E staining demonstrated typical tumor xenografts. B Intracranial tumor volumes in panel A were calculated (mean ± SD, n = 5 for each group, two-tailed Student’s t-test). C GSK3β palmitoylation and GSK3β-STAT3 pathway activity in tumors were detected by western blot. D The mRNA levels of GSC markers (OCT4, NANOG, CD133, and SOX2) in tumor tissues at the end of the experiment were analyzed by RT-PCR. The folding changes were normalized to shNC (mean ± SD, n = 5 for each group, two-tailed Student’s t-test). E U118R cells (shNC and shZDHHC4-2 each in two groups, n = 5/group) were injected into nude mice. Three days after cell injection, mice were intraperitoneally injected with TMZ (25 mg kg−1 d−1) every other day for 30 days. Mice were sacrificed humanely 45 days later. H&E staining demonstrated typical tumor xenografts. F Intracranial tumor volumes in (E) were calculated (mean ± SD, n = 5 for each group, two-tailed Student’s t-test). G The mice were weighed every 4 days (mean ± SD, n = 5 for each group, two-tailed Student’s t-test). H Kaplan–Meier survival curves were used to define the overall survival of intracranial tumor-bearing mice.
