Fig. 1: USP53 was downregulated in HCC and was related to the prognosis of HCC.
From: USP53 plays an antitumor role in hepatocellular carcinoma through deubiquitination of cytochrome c
A USP53 was downregulated in 371 HCC tissues compared to 50 adjacent non-tumor tissues based on TCGA-LIHC dataset. The data was (-LOG10) for USP53 expression(fpkm). The line showed the average value. B, C Kaplan–Meier curves showing the OS and DSS rates of USP53high and USP53low groups. The curves were plotted by Kaplan–Meier Plotter (http://www.kmplot.com). The data was DEseq normalized. D RT-qPCR of USP53 mRNA expression in 33 pairs of HCC and adjacent tissues. E Representative IHC images showing in-situ expression of USP53 in eight pairs of HCC and adjacent non-tumor tissues (magnification 100x and 400x). Scale bar: 50 μm. F, G Immunoblot showing USP53 protein levels in eight pairs of HCC and adjacent peri-tumor tissues. H, I USP53 proteins levels in SMMC-7721, Huh-7, HCCLM3, MHCC97H, MHCC97L, and L02 cell lines. Statistical analysis was based on the difference in the expression levels of HCC cells and L02. *p < 0.05; **p < 0.01; ***p < 0.001.
