Fig. 5: HBP1 methylation alleviates its transcriptional activation of GSN.
From: Methylation of HBP1 by PRMT1 promotes tumor progression by regulating actin cytoskeleton remodeling
A–C RNA-seq analysis found that 16 genes were differentially expressed in HeLa cells transfected with HBP1 or R378A. Heatmap (A) and volcano plot B showed 16 differentially expressed genes between HBP1 and R378A expressing cells with RNA-seq analysis. KEGG enrichment analysis revealed 16 genes enriched in different biological pathways C. D Methylation of HBP1 at R378 alleviates HBP1 promotion of GSN mRNA and protein levels. GSN mRNA and protein levels were measured with real-time PCR and western blotting in HeLa and MGC803 cells transfected with empty vector, HBP1 or R378A. E, F Methylation of HBP1 at R378 alleviates its transcriptional activation on GSN. E HEK293T cells were co-transfected HBP1 or R378A with different GSN promoter segment. The luciferase activities were expressed (right panels). F ChIP assay was used to test HBP1 direct binding to GSN promoter. HEK293T cells were transfected with HBP1 or R378A. Specific binding of HBP1 or R378A to GSN promoter (−400 to −140) were measured by specific PCR (left panel) and real-time PCR (right panel). IgG was used as a negative control. Date were the mean ± SD (n = 3, one-way ANOVA). *p < 0.05, **p < 0.01.
