Fig. 2: DUSP5P1 promotes GC cell migration, invasion in vitro and metastasis in vivo.

A DUSP5P1 was expressed in GC cell lines but not in the normal gastric cell line GES-1 by RT-PCR. B Representative images of migration and Matrigel invasion transwell assay revealed that ectopic expression of DUSP5P1 promoted cell migration (B1), cell invasion (B2) and RFP-tagged GC cell colonies in co-cultures with LO2(B3) in GES1, BGC823, and MGC803 cells, accompanied by enhanced protein levels of β-catenin, Snail, Slug and Claudin-1, and reduced level of E-cadherin (B4). C Representative images of migration and Matrigel invasion transwell assays revealed that knockdown of DUSP5P1 inhibited cell migration (C1) and invasion in MKN74, MGC803 and AGS cells (C2), accompanied by reduced protein levels of β-catenin, Snail, Slug and Claudin-1, and enhanced level of E-cadherin (C3). D Representative macroscopic appearance and histological confirmation by HE staining of lung metastasis of GC cells. DUSP5P1 expression in BGC823 cells significantly increased the number of metastatic lesions in the lungs. E Representative macroscopic appearances of peritoneal surfaces implanting and HE stained images injected with BGC823 cells transfected DUSP5P1 and control vector were shown. F Representative macroscopic appearances of lung metastasis and HE stained images of the lungs injected with MKN45 cells transfected shDUSP5P1 and shNC were shown. G Representative macroscopic appearances of peritoneal surfaces implanting and HE stained images injected with MKN45 cells transfected shDUSP5P1 and shNC were shown. H Representative HE stained images of the liver were shown. Silencing of DUSP5P1 in MKN45 cells significantly reduced the number of metastatic lesions in the liver. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.001.