Fig. 4: ARHGAP5 is the direct downstream target of DUSP5P1.

A Analysis of DUSP5P1 location. A1: DUSP5P1 was mainly located in the nucleus of GC cells by RT-PCR after nuclear and cytoplasmic fractionation. A2: Representative images of nuclear localization of DUSP5P1 in GC tissues by RNA FISH. A Nuclear localization of DUSP5P1 in DUSP5P1 overexpressed BGC823, GES1 and MGC803 cells following DUSP5P1 transfection by RNA FISH. B Flow chart of ChIRP-sequencing assay and RNA-sequencing data analysis of binding site for DUSP5P1 to select the direct downstream target genes of DUSP5P1. C The function and pathway enrichment of DUSP5P1 regulated target genes. D Downstream targets of DUSP5P1 identified by ChIRP-sequencing and RNA-sequencing. D1: The enrichment fold change of even and odd probes by ChIRP-sequencing and D2: the corresponding change of mRNA expression, respectively. E Validation of DUSP5P1 and binding target region of the downstream genes. E1: Representative visual images of enrichment feature of the target regions; E2: ChIRP-PCR was performed to determine the interaction between DUSP5P1 and target region of the downstream genes. F Motif analysis of target sequence by ChIRP-sequencing. G Analysis of motif ARHGAP5 binded. G1: The binding region and the motif analysis of the ARHGAP5 gene. G2: Luciferase reporter assay showed that DUSP5P1 binds to the “TATGTG” motif. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.001.