Fig. 2: Expression of LMNA::NTRK1 fusion in human mesenchymal progenitors (hES-MP). | Oncogenesis

Fig. 2: Expression of LMNA::NTRK1 fusion in human mesenchymal progenitors (hES-MP).

From: Generating in vitro models of NTRK-fusion mesenchymal neoplasia as tools for investigating kinase oncogenic activation and response to targeted therapy

Fig. 2

A Diagram of LMNA::NTRK1 transcript with primers position for RT-PCR. Time course (days) experiment for LMNA::NTRK1 fusion and NTRK1 expression in hES-MP (clones 1 and 3) after Cre expression. B Western blot showing LMNA::TRKA product and phosphorylation of TRKA in hES-MP cells expressing the fusion transcript. C Histogram plot from RNAseq analysis showing NTRK1, NESTIN, S100A and SOX10 RNA levels in hES and hES-MP expressing the LMNA::NTRK1 fusion compared to wild-type isogenic cell line and colorectal cancer cell line KM12 expressing the TPM3::NTRK1 fusion. D GSEA analysis for cell lines expressing the LMNA::NTRK1 fusion (clones 13 and 1.14 (hES-MP) and 13 (hES)). GSEA shows an enrichment of pathways involved in Ewing sarcoma and soft tissue sarcoma.

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