Fig. 2: Olfactory bulb tumors are detectable as early as postnatal day 7 in hGFAP-cre::CrebbpFl/Fl::lsl-MYCN mice.
From: The tumor suppressor CREBBP and the oncogene MYCN cooperate to induce malignant brain tumors in mice
A Histology of brains of P0 mice (CrebbpFl/Fl::lsl-MYCN, n = 3; hGFAP-cre::CrebbpFl/Fl::lsl-MYCN, n = 3) including overview images, high power images and IHC of olfactory bulb of P0 mice. No difference in the number of proliferating cells in the periglomerular layer of the OB was observed (p = 0.6002). B Histology of brains of P7 mice (CrebbpFl/Fl::lsl-MYCN, n = 3; hGFAP-cre::CrebbpFl/Fl::lsl-MYCN, n = 5) including overview images, high power images and IHC of olfactory bulb of P7 mice. The number of proliferating cells in the periglomerular layer of the OB is higher in the mutant (black, p = 0.0724). C Histology of brains of P21 mice (CrebbpFl/Fl::lsl-MYCN, n = 3; hGFAP-cre::CrebbpFl/Fl::lsl-MYCN, n = 4) including overview images, high power images and IHC of olfactory bulb of P21 mice. Extended IHC of olfactory bulb tumors of 3-week-old mice: OLIG2, SOX2, MAP2, GFAP, S100A1, OTX2. The number of proliferating cells in the periglomerular layer of the OB is clearly increased in the mutant (black, p = 0.0103). Boxes mark area of high power images. Arrows mark the tumor. Scale bar corresponds to 2 mm (overview) and 100 μm (high power images). *p < 0.05; ns, p > 0.05; unpaired t tests; vertical bars: SD.
