Fig. 3: hGFAP-cre::CrebbpFl/Fl::lsl-MYCN tumor cells display stem cell features in culture and form tumors in recipient mice.
From: The tumor suppressor CREBBP and the oncogene MYCN cooperate to induce malignant brain tumors in mice
A, B Cultivation of hGFAP-cre::CrebbpFl/Fl::lsl-MYCN tumor cells (B) and control tissue (A) in NSC Medium. N = 5. Scale bar corresponds to 50 μm. C–F High power images and IHC of tumorspheres. Scale bar corresponds to 50 μm. G Viability assay showing in vitro growth of three newly established hGFAP-cre::CrebbpFl/Fl::lsl-MYCN tumor cell lines (nr.1-3) (n = 3, experiments performed in triplicates). Vertical bars: SD. H IHC of in vitro differentiated hGFAP-cre::CrebbpFl/Fl::lsl-MYCN tumor cells. Staining for neural (TUBB3) and glial (GFAP) lineage markers. Scale bar corresponds to 50 μm I Macroscopic image of tumors in recipient mouse brain after tumor cell transplantation. Two of 9 mice developed tumors. J Histological overview image of recipient mouse brain. Scale bar corresponds to 2 mm. K–M High power images and IHC of recipient mouse tumor. Scale bar corresponds to 100 μm. Arrows mark tumors.
