Fig. 5: OB tumors of hGFAP-cre::Crebbp^Fl/Fl::lsl-MYCN mice originate from the V-SVZ stem cell niche.

A Schematic illustration showing the location of the subventricular zone of the lateral ventricle wall (V-SVZ) and the olfactory bulb (OB) in the adult mouse brain. Quiescent neural stem cells (qNSC), actively dividing NSCs (aNSC) and transition-amplifying cells (TAC) are located in the V-SVZ and give rise to neuroblasts (NB) that migrate through the rostral migratory stream (RMS) into the olfactory bulb. B Similarity scores of murine transgene tumors and cell types of the adult ventricular-subventricular zone and olfactory bulb region (V-SVZ/OB) in the GCERT2 fate mapping model (ref. [23]). Similarity was calculated by logistic regression, either for all tumor cells together (left), or separately for individual clusters (right). Colors represent the probability of high (red) to low (gray) similarity. C, D UMAP plots showing tissue and cluster distribution of GCERT V-SVZ/OB cells (C), of hGFAP-cre::Crebbp^Fl/Fl::lsl-MYCN cells (D), and expression of marker genes for aNSC (Hes6), TAC (Ube2c), and NB (Stmn2) in both datasets. Arrowheads mark cell populations with the highest log2 expression values.