Fig. 7: Cells with genetic modification of locus 22 and silence of MAFG or NEF2L1 are failed to escape the T cell-mediated killing. | Oncogenesis

Fig. 7: Cells with genetic modification of locus 22 and silence of MAFG or NEF2L1 are failed to escape the T cell-mediated killing.

From: Identifying a locus in super-enhancer and its resident NFE2L1/MAFG as transcriptional factors that drive PD-L1 expression and immune evasion

Fig. 7

A Representative images of SUM-159 cell before and after co-cultured with activated T cells. Representative images of activated T cells co-cultured with SUM-159 cells or genetic modified cells (sgVector, sg-22, sg-MAFG-1, sg-MAFG-2, sg-NFE2L1-1 and sg-NFE2L1-2; B Quantification of remaining SUM-159 cells and its genetic modified cells after co-cultured with activated T cells; C Real-time PCR was performed to examine the relative mRNA levels of Granzyme B in activated T cells co-cultured with control or genetic modified cells; D Real-time PCR was performed to examine the relative mRNA levels of IFNγ in activated T cells co-cultured with control or genetic modified cells; E The expression of MAFG, MAFF, MAFK, NFE2L1, NFE2L2, CD273 and CD274 in breast cancer cell lines. F The expression of MAFG is positively correlated with PD-L1 and PD-L2 in breast cancer cell lines. Data from (A) and (B) are representative of three independent experiments. Data from (C) and (D) are representative of two independent experiments. *p < 0.05; **p < 0.01, ***p < 0.001.

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