Fig. 6: DDX3X binds to SIRT7.
From: DDX3X interacts with SIRT7 to promote PD-L1 expression to facilitate PDAC progression
A Intersection between our mass spectrometry-identified set of DDX3X binding proteins and reported mass spectrometry-identified sets of SIRT7 binding proteins. B The interaction of SIRT7 (purple) with DDX3X (gold) predicted by computational protein‒protein docking. C An analysis utilizing coimmunoprecipitation was conducted to evaluate the impact of SIRT7 on the interaction with DDX3X in PANC-1 cells. D The protein level of SIRT7 in PC cells was examined using Western blot analysis following knockdown of DDX3X. E Expression of SIRT7, CD274, NEM1, COPS2, RPS20, RPS14 and RPS7 was checked in DDX3X-overexpressing PANC-1 cells. F Representative fluorescent multiplexed immunohistochemical staining (MIF) sections of DDX3X (red) and SIRT7 (green) expression and distribution in PDAC patient tissues and KPC mouse tissues. G Representative fluorescent multiplexed immunohistochemical staining (MIF) sections of DDX3X (red) and SIRT7 (green) expression and distribution in subcutaneous xenograft tumors and orthotopic xenograft tumors.
