Fig. 3: LATS1/2 KO-driven YAP/TAZ activation suppresses indisulam-induced alternative splicing.
From: YAP/TAZ interacts with RBM39 to confer resistance against indisulam
a Schematic representation of the PCR products of TRIM27 detecting alternative splicing. b Alternative splicing of TRIM27 in HNSCC cell lines. c Alternative splicing of TRIM27 in WT and LATS1/2 KO CAL27 cells treated with indisulam. (d) Alternative splicing treated with E7820. (e) Relative mRNA expression of CTGF and DCAF15 in WT and LATS1/2 KO CAL27 cells (N = 3). (f) Comprehensive splicing analysis of DMSO or indisulam-treated WT and LATS1/2 KO CAL27 cells. SE skipped exon, A5SS alternative 3’ splice site, A3SS alternative 5’ splice site, MXE mutually excluded exon, RI retained intron. (g) GO and KEGG pathway enrichment analysis of the SE genes. Cell cycle-related are highlighted in green, and DNA-metabolism related are in blue. Student’s t-test was used. Mean ± SEM (e); ****, P < 0.0001; ***, P < 0.001; **, P < 0.01.
