Fig. 4: In vitro efficacy of combined PI3K and STAT3 inhibition.
From: Combined Inhibition of PI3K and STAT3 signaling effectively inhibits bladder cancer growth
A The synergistic score between copanlisib and napabucasin was determined using Loewe and HSA synergy analysis in six BCa cells. Representative images (B) and quantification (C) of apoptosis assay in six BCa cells treated with DMSO, copanlisib, napabucasin or combination respectively. D Representative images of Live/Dead staining assay in organoid models treated with DMSO, copanlisib, napabucasin or combination respectively. E Organoids were treated with DMSO, copanlisib, napabucasin or combination in a CellTiter-Glo 3D cell viability assay. F Population doublings of 5637 and UM-UC-3 cells treated with DMSO or copanlisib and napabucasin for 20 days. Data are presented as mean ± SD of independent samples with individual data points shown; P values were assessed by two-tailed Student t test in comparison with single-agent cells group (C, E); *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.
