Fig. 2: P4HB inhibition impedes GSCs progression and oncogenicity by inducing apoptosis. | Oncogenesis

Fig. 2: P4HB inhibition impedes GSCs progression and oncogenicity by inducing apoptosis.

From: P4HB maintains Wnt-dependent stemness in glioblastoma stem cells as a precision therapeutic target and serum marker

Fig. 2

A–C Cell proliferation assay for X01, 448, and 83 GSCs infected with shCtrl, shP4HB-1, and shP4HB-2 lentiviral vectors. Data are presented as means ± SD, n = 3, *P < 0.05, ***P < 0.001, t-test. D Kaplan–Meier survival curves of mice implanted with 83 GSCs infected with shCtrl or shP4HB lentivirus (n = 6 in each group, 1 × 104 GSCs/mouse), P < 0.0001, log-rank test. E H&E staining of the whole brain slides derived from the mice implanted with 83 GSCs infected with shCtrl or shP4HB lentivirus. F–H Flow cytometry analysis of Annexin V/PI staining for cellular apoptosis analysis of X01, 448, 83 GSCs infected with shCtrl or shP4HB lentivirus. I–K Western blot analysis of PARP, C-PARP, P4HB, Caspase-3, CC3, and BCL2 protein levels in X01, 448, 83 GSCs infected with shCtrl or shP4HB lentivirus, α-tubulin was used as a loading control.

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