Fig. 3: CENPF regulates TERT to promote progression of early-stage LUAD.
From: CENPF (+) cancer cells promote malignant progression of early-stage TP53 mutant lung adenocarcinoma
A intersection analysis of DEGs in two CENPF-KD models of PC-9 cells (sh-NC/CENPF lentivirus); Proliferative ability examined by CCK-8 assay in CENPF-KD model with TERT overexpression of PC-9 (B) and HCC827 (C) cell lines (sh-NC/CENPF lentivirus; pc-NC/TERT plasmids); Detection of markers for stem cells in CENPF-KD model with TERT overexpression of PC-9 (D) and HCC827 (E) cell lines (sh-NC/CENPF lentivirus; pc-NC/TERT plasmids). F Protein content of CENPF, TERT and Ki-67 by IHC detected in tumors of in autochthonous mouse models of LUAD driven by EML4-ALK treated with AAV6-sh-NC/CENPF. Correlation between CENPF and TERT in LUAD (G) and stage IA LUAD (H). I Combined OS rate analysis of CENPF and TERT in five LUAD datasets. J IP of CENPF and histone H3. K Epigenetic modifications of H3 histone examined by ChIP assay in CENPF-KD model of PC-9 cell lines. *P < 0.05; **P < 0.01; ***P < 0.001. N ≥ 3. Data are presented as mean ± SD. Multi-way ANOVA with Tukey’s multiple comparisons test (B, C). One-way ANOVA with Dunnett’s t test for multiple comparisons test (D, E). Pearson correlation analysis (G, H). The chi-square test (I). Two-group t test (K). CENPF centromere protein F, TERT telomerase reverse transcriptase, LUAD lung adenocarcinoma, DEGs differentially expressed genes, KD knockdown, IHC immunohistochemistry, NC negative control, EML4-ALK echinoderm microtubule-associated protein-like 4 and anaplastic lymphoma kinase, AAV6 Adeno-associated virus serotype 6, OS overall survival, IP immunoprecipitation, WB western blot, ChIP chromatin immunoprecipitation.
