Fig. 2: EPHA5 promote proliferation and inhibit apoptosis in FTC cells.

A Western blot analysis of EPHA5, PCNA, Cyclin D1, Caspase-3, Cleaved-caspase-3, and β-actin in FTC-133 and FTC-238 cells. **p < 0.01, n = 3. B, C CCK-8 assay analysis of FTC-133 and FTC-238 cells transfected with si-NC or si-EPHA5 respectively. **p < 0.01, n = 6. D Representative TUNEL staining of FTC cells; the nuclei were stained with DAPI. Scale bar, 200 μm. TUNEL scores. The relative number of apoptotic cells is represented as TUNEL-positive cells/DAPI. **p < 0.01, n = 6. E Colony formation assay of FTC-133 and FTC-238 cells transfected with si-NC or si-EPHA5 respectively. **p < 0.01, n = 6.