Fig. 6: Higher SOX11 expression correlates with shorter survival times in colon and rectum adenocarcinomas.

A The gene expression matrices of colon adenocarcinoma (COAD) and rectum adenocarcinoma (READ) samples from the TCGA database were retrieved from the UCSC Xena browser, and SOX11 RNA expression values in combined COAD and READ cohorts were visualized as box plots. Black dots show SOX11 expression in individual samples. The box plots show the 26th to 75th percentiles of the data and the median. Statistical analyses were performed using Mann–Whitney U-test, ns: not significant. B COAD and READ gene expression matrices from the TCGA database were stratified according to tumor stages, and SOX11 expression levels in combined COAD and READ stage I, II, III, and IV primary tumors are displayed. Black dots show SOX11 expression in individual samples. Mann–Whitney U test, ns: not significant; *: p value < 0.05; **: p value < 0.01; ***: p value < 0.001. C Kaplan-Meier plots showing overall survival and disease-free interval associated with high [log₂(FPKM-UQ + 1) > 3.842] and low [log₂(FPKM-UQ + 1) < 3.842] SOX11 expression in TCGA COAD and READ cohorts. The p values were calculated by the log-rank method. D Correlation analyses of the expression of the genes indicated in combined transcriptomes of COAD and READ primary tumors. Each orange dot shows an individual sample. A linear regression line (red line), rank-based Spearman correlation coefficients, and p values are annotated in each plot. The pink shaded regions indicate 95% confidence intervals. Gene expression levels are given as log₂(FPKM-UQ + 1) values. E Box plots visualizing expression of the genes indicated in CMS-stratified transcriptomes of COAD and READ primary tumors from the TCGA database. Each black dot shows an individual sample. The box plots show the 26th to 75th percentiles of the data and the median. Statistical analyses were performed using Mann–Whitney U-test; ***: p value < 0.001. NC not classifiable.