Abstract
Background
To identify inflammatory cell types by phenotypic analysis of the inflammatory cells in the induced sputum.
Methods
This retrospective study included 1232 children and infants, who were assigned into mild/moderate groups (326) and severe group (602) by clinical symptom scores. Phenotypes of sputum inflammatory cells were analyzed using liquid-based thin-cytologic test and eosinophil-derived neurotoxin (EDN) was quantified by ELISA.
Results
Blood eosinophil count, serum total IgE level, and allergen detection rate were significantly higher in the severe group. In the 905 cases of qualified sputum, 526 cases exhibited at least one type of inflammatory cells, including neutrophil (343, 65.2%), eosinophil (161, 30.6%), and mixed granulocytes (22, 4.2%). Levels of neutrophils and eosinophils were significantly higher in the severe group than mild/moderate group, and eosinophil was predominant in the severe group. Serum EDN was 104.8 ± 39.4 μg/l in the eosinophil phenotype group, 112.6 ± 41.2 μg/l in the mixed group, 88.2 ± 36.6 μg/l in the neutrophil phenotype group, and 60.9 ± 34.6 μg/l in the paucigranulocytic phenotype group.
Conclusion
Induced-sputum inflammatory cell count may be used to determine phenotype of wheezing. The criteria of classifying adult asthma could be applicable for children and infants.
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Acknowledgments
This study is supported by the key discipline project of Tianjin health and family planning commission (13KG125).
Author contributions
Y.Z. contributed to the study design, Y.G. and J.Z. prepared the manuscript, J. Li and J. Liu performed the data analysis, C.M. searched references, X.J. and L.Z. collected the data. All the authors made the final approval.
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The datasets supporting the conclusions of this article are included within the article.
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The authors declare no competing interests.
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The study protocol was approved by the Ethic Committee of Tianjin Children’s Hospital and conducted in accordance with Helsinki’s Declaration. All the patients gave their written information consent.
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Guo, Y., Zou, Y., Zhai, J. et al. Phenotypes of the inflammatory cells in the induced sputum from young children or infants with recurrent wheezing. Pediatr Res 85, 489–493 (2019). https://doi.org/10.1038/s41390-018-0268-5
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DOI: https://doi.org/10.1038/s41390-018-0268-5
