Fig. 3: SAG increases oligodendrocyte proliferation but not differentiation following tMCAO.

a Representative images showing proliferating cells (BrdU+) in the corpus callosum (CC) positive for Olig2 in rats given tMCAO administered with saline (left) or SAG (right) at P24. Scale bar, 50 μm. Inset, magnified view of single-channel immunostains. Arrows denote double-labeled cells. b Quantification of cells double-labeled with BrdU and Olig2 in the CC. Black bars, CC from ipsilateral hemisphere; white bars, CC from contralateral hemisphere. c Quantification of total Olig2+ cells in the CC in the ipsilateral hemisphere. d Representative images showing Olig2+ cells double-labeled with Nkx2.2 in the CC in rats given tMCAO administered with saline (left) or SAG (right) at P24. Scale bar, 50 μm. Inset, magnified view of single-channel immunostains. Arrows denote double-labeled cells. e Proportion of Nkx2.2+/Olig2+ cells in the CC of the ipsilateral hemisphere in tMCAO+SAG vs tMCAO+Veh. f Representative images of the ipsilateral hemispheres stained for MBP (green) in P24 rats (left), and insets shown at higher magnification (right). Scale bar, 500 μm. g Representative images showing Olig2+ cells double-labeled with CC1 in the CC in rats given tMCAO administered with saline (top) or SAG (bottom). Scale bar, 20 μm. h Quantification of CC1+/Olig2+ cells in the CC of the ipsilateral hemisphere in tMCAO+SAG vs tMCAO+Veh. *p < 0.05, one-way ANOVA with Tukey’s multiple comparisons test.