Fig. 4: SAG promotes oligodendrogenesis and maturation in an adult rat model of focal demyelination.
From: Neuroprotective effects of Sonic hedgehog agonist SAG in a rat model of neonatal stroke
a Schematic of EB model. b Experimental timeline for focal demyelination using ethidium bromide (EB) injection into the rat cerebellar peduncle (CCP). c Representative images of proliferating cells (Ki67, red) and OPCs (Nkx2.2, white) stained within the lesioned area (dotted lines) at 7 days post injury (dpi). Scale bar, 25 μm. Inset, magnified view. Scale bar, 10 μm. Arrowheads denote cells double-positive for Ki67 and Nkx2.2. d Quantification of Nkx2.2+/Ki67+ cells (top), and total Nkx2.2+ cells (bottom) from lesioned areas after vehicle or SAG administration. *p < 0.05, Student’s t test.
