Fig. 5: SAG enhances angiogenesis following tMCAO.
From: Neuroprotective effects of Sonic hedgehog agonist SAG in a rat model of neonatal stroke
a Cells in the injured core (striatum) stained with BrdU and Collagen 4A (Col4A), an EC marker, to visualize proliferation of cells associated with the vasculature. Arrows denote BrdU+ cells in direct contact with Col4A+ vessels. Scale bar, 100 μm. Right, diagram depicting area of analysis. b Cells co-stained with Olig2 and Col4A in the striatum. Arrows denote Olig2+ cells in contact with Col4A+ vessels. c Cells co-stained with Col4A and Claudin5, a marker of blood–brain barrier (BBB) structure. d Quantification of BrdU+/Col4A+ cells in the ipsilateral striatum per mm3. e Quantification of vessel area from Col4A-labeled vasculature in the motor and somatosensory cortical regions bordering the striatal core. f Quantification of vessel junction number per mm3 in the same region as e. *p < 0.05, one-way ANOVA with Tukey’s multiple comparisons test.
