Table 4 Primary outcomes of the included studies.
Reference | Functional outcome | Participation | Survival of the animals | ||||||
|---|---|---|---|---|---|---|---|---|---|
Motor performance | Cognitive performance | Sensory function | Social interaction | ||||||
Test | ↑↓↔ | Test | ↑↓↔ | Test | ↑↓↔ | Test | ↑↓↔ | ||
Cho et al. (2016);34 Korea | Rotarod test | 2 weeks post MSC: ↔; 8 weeks post MSC: ↑ only MSC-EE group | NR | NR | NR | NR | |||
Grip strength test | 2 weeks post MSC: ↔; 8 weeks post: ↑ only MSC-EE group | ||||||||
Ding et al. (2015)22 | NR | Morris water maze | 21 days post injury: ↑ | NR | NR | NR | |||
Ding et al. (2017)23 | Hanging wire | 16, 22, and 28 days post injury: ↑ | NR | NR | NR | NR | |||
Vertical pole test | 16, 22, and 28 days post injury: ↑ | ||||||||
Kim et al. (2012);35 Korea | Rotarod test | 26 and 27 days post injury: ↔; 28 days post injury: ↑ | NR | NR | NR | Survival at 28 days post injury; Sham control group =100%; MCAO-MC (vehicle) group = 40%; MCAO-MM (MSCs) group = 83% | |||
Cylinder test | 28 days post injury: ↔ | ||||||||
Larpthaveesarp et al. (2021)37 | Cylinder test | P61, P62 postnatal:; ↑ MACO+7 d MSC; ↔ MACO+3 d MSC | Novel object recognition | P54: ↔ ↑only for MSC/EPO and EPO | MCAO+Vehicle group = 89%; 3 d MCAO+MSCs = 100%; 7 d MCAO+MSCs = 80% | ||||
Open field test | P59: ↔ | ||||||||
Lee et al. (2010);36 Korea | Rotarod test | 14, 20, 30, and 40 days post MSCs: ↔ | NR | NR | NR | NR | |||
Cylinder test | Overall: ↑; 14 days post MSCs: ↔; 20 days post MSCs: ↑ | ||||||||
Li et al. (2014);24 China | Shuttle box test | 1–5 days post MSCs: ↔ | NR | NR | NR | ||||
Ohshima et al. (2015);40 Japan | NR | NR | NR | NR | NR | ||||
Sakai et al. (2018);41 Japan | Beam walk test | 25 days post MSCs: ↑ | NR | NR | NR | NR | |||
van Velthoven et al. (2017);38 USA | Cylinder rearing test | 28 days post injury: ↑ | NR | NR | NR | The survival rate at the end of the study is unclear; before the randomization of the treatment groups and 3 days after tMCAO the survival rate is reported to be 87% | |||
van Velthoven et al. (2013);33 Netherlands | Cylinder rearing test | 14, 21, and 28 days post injury: ↑ at all timepoints | NR | NR | NR | Unclear by the end of the study; 87% post-MCAO and prior to randomization to the treatment groups | |||
Adhesion removal test | 28 days post injury: ↑ | ||||||||
van Velthoven et al. (2010);29 Netherlands | Cylinder rearing test | 10, 21, and 18 days post injury: ↑ MSC-3: versus VEH; 21 and 28 days post injury: ↑ MSCS-3+10: versus VEH, versus MSC-3 | NR | NR | NR | Approx. 90% post HI insult | |||
Rotarod treadmill | 21 days post injury: ↑MSC-3, MSC-3+10 versus VEH 28 days: ↑MSC-3, MSC-3+10 versus VEH↑ MSC-3+10 versus MSC-3 | ||||||||
van Velthoven et al. (2010);31 Netherlands | Cylinder rearing test | 21 and 28 days post injury: ↑ versus VEH | NR | NR | NR | Approx. 90% post HI insult | |||
van Velthoven et al. (2010);32 Netherlands | Cylinder rearing test | 10 and 21 days post injury: ↑ versus VEH | NR | NR | NR | Approx. 90% post HI insult | |||
van Velthoven et al. (2012);30 Netherlands | Cylinder rearing test | 21 and 28 days post injury: ↑ versus VEH | NR | NR | NR | NR | |||
Wei et al. (2015);39 USA | Adhesion removal test | 10, 17, and 24 days post injury: ↑ versus saline | NR | Modified buried food-finding test | 17 and 24 days after injury: ↑ | Social interaction test | 17 and 24 days after injury: ↑ versus Saline | NR | |
Home-cage activity | 17 and 24 days after injury: ↑ versus saline | ||||||||
Xia et al. (2010);25 China | Modified neurological severity score: muscle status, abnormal movement Beam balance test reflex absence | 14, 21, and 20 days post MSCs: ↑ overall score | NR | Modified neurological severity score: sensory tests | 14, 21, and 20 days after MSCs: ↑overall score | NR | NR | ||
Yang et al. (2020)28 | NR | Spatial version of the Morris water maze task | 4 weeks post injury: ↑ | NR | NR | NR | |||
Zhang et al. (2014);26 China | Longa scoring | 6 h and 7 and 20 days post injury: ↑ | Morris water maze task | 4 weeks post injury: ↑ MSC-24 h versus MSC-72 h and VEH-24 h | NR | NR | NR | ||
Rotarod testing | 20 days post injury: ↑ | ||||||||
Zhou et al. (2015)27 | NR | Morris water maze test; object-in-place task | 4 weeks post MSC:↑; 6 weeks old animals: ↑ | NR | NR | NR | |||
