Fig. 2: In neonates with NEC, Tregs are less proliferative and actTregs express a lower level of FOXP3 compared to controls with feeding intolerance, at 7–10 days post birth.
From: Reduction in regulatory T cells in preterm newborns is associated with necrotizing enterocolitis
a, b Representative plots (a) and cumulative analysis (b) showing the percentages of Ki67+ cells in gated CD127low CD25high Tregs from the peripheral blood of preterm neonates with NEC (n = 6), feeding intolerance (FI, n = 19) or feeding tolerance (FeedTol, n = 28), collected at 0–3 (T0), 7–10 (T1), or 28–31 (T2) days post birth. Numbers indicate the percentages of Ki67+ cells. c, d Representative histograms (c) and cumulative analysis (d) showing FOXP3 expression in actTreg population in the same samples as above. Numbers in the histogram plots indicate the gMFI (geometric mean fluorescence intensity) of FOXP3 expression in gated CD45RAlow FOXP3high actTregs in the neonate subgroups as indicated in the legends. *p < 0.05, by Mann–Whitney test. In each analysis, only data from neonates whose samples were available at least at both T0 and T1 were included.
