Fig. 3: Fluorescent microphotographs of GFAP immune-stained samples from the piglet SVZ after HI and normothermia (38.5 °C) or HT at 35, 33.5 or 30 °C. | Pediatric Research

Fig. 3: Fluorescent microphotographs of GFAP immune-stained samples from the piglet SVZ after HI and normothermia (38.5 °C) or HT at 35, 33.5 or 30 °C.

From: Therapeutic hypothermia modulates the neurogenic response of the newborn piglet subventricular zone after hypoxia-ischemia

Fig. 3

a GFAP reveals the length of the processes of radial-glia/neural stem cells in the SVZ. The length of GFAP+ processes after HI and normothermia (38.5 °C) was shorter when compared with HT at 33.5 °C (**p < 0.01 in the L-SVZ and DL-SVZ) and at 35 °C (*p < 0.05 in the DL-SVZ and L-SVZ). HT at 30 °C obtained lower values (‡p < 0.01) than 33.5 °C in both the roof and the L-SVZ. b Quantification of GFAP expression using densitometry. The highest values of GFAP coverage (expressed as GFAP % area in the graphs) were observed in 33.5 °C animals for the three areas evaluated; however, there were no significant differences in GFAP coverage between normothermia (38.5 °C) and cooling to any temperature. HT at 30 °C obtained significant lower values (†p < 0.05) than 33.5 °C in the L-SVZ. LV, lateral ventricle. Original magnification 400x. Scale bar: 100 µm.

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