Fig. 3: TLR4 regulates the effect of postbiotics from B. infantis on F-actin protein expression.
From: Synergism between TLR4 and B. infantis in the development of the premature intestine
H4 cells transfected with TLR4 siRNA or control siRNA were seeded onto chamber slides and treated with or without postbiotics from B. infantis. F-actin was detected by IF staining (green), the nuclei were counterstained with Dapi (blue) (a). The images are representative of 3 independent experiments. The analysis applied by corrected total cell fluorescence (CTCF) with image J (b). One-way ANOVA and post hoc tests were used for statistic-analysis, *P < 0.05, ***P < 0.001. Amplified x 400; scale bar = 100 pixels; n= 10–17 cells/treatment.
