Fig. 5: Representative microphotographs of cleaved caspase-3 immunostaining and hematoxylin counterstain of the periventricular white matter from female and male piglets showing cleaved caspase-3 positive cells (black arrows).
From: Sex dimorphism in brain cell death after hypoxia-ischemia in newborn piglets
Original magnification: ×400. Scale bar: 100 μm. Graphs: a Averaged number of cleaved caspase-3 positive cells per mm2 (pooled across regions and bregma 0/−2.0 levels) from females and males. The overall number of cleaved caspase-3 positive cells per mm2 was significantly (Unpaired t-test, ****p < 0.0001) lower in males versus females. AVG average. b Quantitative results of regional evaluation of cleaved caspase-3 positive cells between females and males. cTEX cingulate cortex, sTEX sensorimotor cortex, PvWM periventricular white matter, CDT caudate nucleus, THAL thalamus. Unpaired t-test for sTEX and CDT; Mann-Whitney for cTEX, PvWM and THAL. *p < 0.05 or **p < 0.01 vs females.
